Ammonia-Ammoniun Quantification Assay

 

AMMONIA (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems. Ammonia is found in the atmosphere, rainwater, soil, seawater, volcanic areas. It is widely used as fertilizer, cleaner, antimicrobial agents and in fermentation and chemical synthesis. This convenient assay uses o-phthalaldehyde reagent to directly measure NH3/ NH4+. The fluorescence intensity measured using handheld fluorometer at ex/em = 360/460nm is directly proportionate to the NH3 concentration in the sample.

  

 

➥ Samples: Urine, soil extracts, swimming pool samples etc.

 

➥ Assay Performance:

● Linear Detection Range: 0 to 1 mM (0 – 17ppm).
● Detection Limit: 30 μM (0.5 ppm).
● Typical Precision (CV%): <2% at 1mM; <4% at 0.25 mM.

 

➥ Product Information:
● Ammonia Assay Kit: sufficient for approximately 200 assays. Kit content: 1 mL Reagent A; 1 mL Reagent B; 20 mL Assay Buffer, 400 μL Standard.
● Mini glass tubes (optional).
● Fluorometer comes with a 5VDC power adapter, a USB cable, manual and data management software CD.
* Avoid contact and inhalation. Standard laboratory safety procedures should be followed when handling this product. Safety procedures include wearing OSHA approved safety glasses, gloves and protective clothing.
* Shipping and storage: the kit is shipped at room temperature. Store Reagents A and B at -20°C, Assay Buffer and Standard at 2-8°C. Shelf life: 12 months after receipt.

 

➣➣ See the detailed information: Ammonia/Ammonium Quantification with Fluorometer 360/460nm